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Updated: Mar 5, 2026

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

Plasmonics Meets Biology through Optics.

Luciano De Sio¹, Giulio Caracciolo², Ferdinanda Annesi³

¹Beam Engineering for Advanced Measurements Company, Winter Park, FL 32789, USA. luciano@beamco.com.

Nanomaterials (Basel, Switzerland)

|March 29, 2017

Summary

Researchers characterized gold nanorods (GNRs) and DNA interactions. They identified optimal conditions for a GNRs/DNA complex, enabling sensitive, non-invasive optical monitoring of temperature changes for potential therapeutic applications.

Keywords:

DNA nanomaterials optics plasmonics

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Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers

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Published on: March 21, 2025

Area of Science:

Nanomaterials Science
Biophysics
Plasmonics

Background:

Plasmonic metallic nanoparticles (NPs) offer nanoscale light localization via Localized Plasmon Resonance.
These NPs show therapeutic potential in triggering DNA release and enhancing tissue ablation while sparing healthy tissue.
Understanding NP-biomolecule interactions is crucial for developing advanced biomedical applications.

Purpose of the Study:

To characterize the electrostatic interaction between positively charged gold nanorods (GNRs) and negatively charged whole-genome DNA.
To determine the optimal conditions for forming a stable and effective GNRs/DNA hybrid biosystem.
To investigate the photo-thermal properties of the resulting GNRs/DNA complex for potential non-invasive monitoring applications.

Main Methods:

ζ-potential measurements to analyze surface charge and stability.
Hydrodynamic diameter analysis to assess particle size and complex formation.
Gel electrophoresis to confirm successful complexation and evaluate stability.

Main Results:

The study identified specific conditions for creating a promising GNRs/DNA complex based on DNA concentration.
Characterization revealed the electrostatic interaction dynamics between GNRs and DNA.
The photo-thermal properties of the optimized GNRs/DNA complex were successfully investigated.

Conclusions:

A hybrid biosystem combining plasmonic nanoparticles and DNA can be effectively designed.
The GNRs/DNA complex exhibits properties suitable for advanced biomedical applications.
This research paves the way for non-invasive, all-optical methodologies for monitoring photo-induced temperature variations with high sensitivity.