Detoxification of hexavalent chromate by growing Paecilomyces lilacinus XLA

In the study, the capability of Paecilomyces lilacinus XLA (CCTCC: M2012135) to reduce Cr⁶⁺ and its main antagonistic mechanisms to Cr⁶⁺ were experimentally evaluated. Activated growing fungus XLA efficiently reduced over 90% Cr⁶⁺ in the media with Cr⁶⁺ concentration below 100 mg L^-1 at pH 6 after 14 days. After 1-day exposure to 100 mg L^-1 Cr⁶⁺, nearly 50% of Cr⁶⁺ was reduced. Moreover, SO₄^2- stimulated Cr⁶⁺ reduction, whereas other interferential ions inhibited Cr⁶⁺ reduction. The interaction mechanisms between XLA and Cr⁶⁺ mainly involve biotransformation, biosorption, and bioaccumulation, as detected by electron microscopy and chemical methods. The lower concentrations of Cr⁶⁺ (5 and 50 mg L^-1) stimulated the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) level in XLA, respectively, but the higher concentration of Cr⁶⁺ (150 mg L^-1) decreased the enzymatic activities and GSH concentration. The results implied that SOD, CAT and GSH were defensive guards to the oxidant stress produced by Cr⁶⁺. All these extracellular/intracellular defense systems endowed XLA with the ability to resist and detoxify Cr⁶⁺ by transforming its valent species. The fungus XLA could efficiently reduce Cr⁶⁺ under different environmental conditions (pH, interferential ions, and concentration). Moreover, XLA could endure the high concentration of Cr⁶⁺ probably due to its high biotransformation capability of Cr⁶⁺ and intracellular antioxidant systems for the detoxification of ROS generated by external Cr⁶⁺. All these results suggested that the fungus XLA can be applied to remediation of Cr⁶⁺-contaminated environments.