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Live imaging of root-bacteria interactions in a microfluidics setup.

Hassan Massalha1, Elisa Korenblum1, Sergey Malitsky1

  • 1Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot 76100, Israel.

Proceedings of the National Academy of Sciences of the United States of America
|March 29, 2017
PubMed
Summary

A new microfluidics system called TRIS allows real-time imaging of plant root-microbe interactions. This technology reveals bacterial chemotaxis and colonization patterns, offering insights into rhizosphere microbial ecology.

Keywords:
Bacillus subtilisTRISlive-imaging microscopymicrobial community dynamicsroot–bacteria interaction

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Area of Science:

  • Microbial Ecology
  • Plant Science
  • Biotechnology

Background:

  • Plant roots create a unique rhizosphere environment crucial for microbial interactions.
  • Current methods for studying root-microbe dynamics at high resolution are complex and limited.

Purpose of the Study:

  • To develop and validate a microfluidics-based system for real-time, high-resolution imaging of root-bacteria interactions.
  • To investigate the chemotactic behavior and colonization dynamics of specific bacteria on plant roots.

Main Methods:

  • Development of the Tracking Root Interactions System (TRIS), a microfluidic device with multiple parallel chambers.
  • Monitoring fluorescently labeled *Bacillus subtilis* and *Escherichia coli* interactions with *Arabidopsis thaliana* roots in real time.
  • Utilizing dual inoculation and multi-genotype root tracking capabilities within the TRIS device.

Main Results:

  • Observed distinct chemotaxis of *B. subtilis* towards the *Arabidopsis thaliana* root elongation zone.
  • Demonstrated rapid colonization of the elongation zone by *B. subtilis* within 6 hours.
  • Showed active exclusion of *E. coli* by *B. subtilis*-colonized roots, suggesting a protective role.

Conclusions:

  • The TRIS microfluidics device offers unprecedented insights into root microenvironment microbial ecology.
  • This system facilitates real-time monitoring of bacterial behavior and plant-microbe interactions.
  • TRIS is expected to accelerate discoveries in plant-microbe interactions and rhizosphere research.