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Calpain II involvement in mitosis.

J E Schollmeyer1

  • 1U.S. Department of Agriculture, Roman L. Hruska Meat Animal Research Center, Clay Center, NE 68933.

Science (New York, N.Y.)
|May 13, 1988
PubMed
Summary

Calcium-activated protease calpain II (a ubiquitous enzyme) relocates during mitosis, suggesting its role in cell division. Its injection accelerates mitotic spindle disassembly and anaphase onset.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Mitotic spindle disassembly involves cytoskeletal changes.
  • Mitosis is associated with calcium (Ca2+) transients.
  • The role of Ca2+-activated protease calpain II in mitosis is unknown.

Purpose of the Study:

  • To investigate the involvement of calpain II in the mitotic process.
  • To determine the localization of calpain II during mitosis.

Main Methods:

  • Synchronized PtK1 cells were used.
  • Immunofluorescence monitored calpain II relocation.
  • Microinjection of calpain II was performed.

Main Results:

  • Calpain II relocated from the plasma membrane to mitotic chromosomes, perinuclear regions, and the mid-body during mitosis.
  • Microinjection of calpain II induced metaphase onset.
  • Injection at late metaphase triggered premature spindle disassembly and anaphase.

Conclusions:

  • Calpain II is involved in mitosis.
  • Calpain II localization changes dynamically during cell division.
  • Calpain II may regulate mitotic spindle dynamics.

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