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Updated: Feb 3, 2026

A FACS-based Protocol to Isolate RNA from the Secondary Cells of Drosophila Male Accessory Glands
Published on: September 5, 2019
Drosophila Male Meiosis
Silvia Bonaccorsi1, Maurizio Gatti2,3
1Department of Biology and Biotechnology "Charles Darwin", Sapienza, University of Rome, P.le. A Moro 5, 00185, Rome, Italy.
Abstract:
In Drosophila males, there is no synaptonemal complex and recombination does not occur. Thus, Drosophila male meiosis is a good model system for the analysis of achiasmate chromosome segregation. In addition, due to their large size, the meiotic spindles of Drosophila males are an excellent system for mutational dissection of the mechanisms of spindle assembly. Here, we describe the main techniques for visualization of live Drosophila testes and for preparation of fixed meiotic chromosomes and spindles.
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