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Updated: Mar 5, 2026

A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress

A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress

Published on: March 21, 2014

Leukocyte Adhesion Under Hemodynamic Flow Conditions.

Charlotte Lawson¹, Marlene Rose², Sabine Wolf³

¹Comparative Biomedical Sciences, Royal Veterinary College, Royal College Street, London, NW1 0TU, UK. chlawson@rvc.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)

|March 29, 2017

Summary

This study introduces a new in vitro model to investigate how fluid shear stress affects vascular endothelial cells (ECs) and their interactions with leukocytes during prolonged co-culture.

Keywords:

Endothelium Leukocyte Parallel-plate flow chamber Shear stress

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Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay

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Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay

Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay

Published on: December 30, 2014

Area of Science:

Biomedical Engineering
Cell Biology
Physiology

Background:

Vascular endothelial cells (ECs) form a barrier between blood and tissue, responding to stimuli like fluid shear stress.
Current research often uses static cultures, limiting understanding of ECs under dynamic flow conditions.
Understanding EC function under shear stress is crucial for vascular health and disease research.

Purpose of the Study:

To present a novel in vitro system for studying endothelial cell (EC) function under shear stress.
To enable investigation of prolonged leukocyte-endothelial cell interactions in a flow environment.
To provide a methodology for assessing the impact of shear stress on both ECs and leukocytes.

Main Methods:

Development of an in vitro system for recirculating cell culture medium and leukocytes over an endothelial cell layer.
Application of controlled fluid shear stress to the endothelial cells.
Prolonged co-culture of leukocytes with endothelial cells for minutes to days.
Analysis of endothelial and leukocyte populations following co-culture under flow.

Main Results:

The described system effectively simulates physiological fluid shear stress on endothelial cells.
Prolonged co-culture under flow allows for dynamic observation of leukocyte-endothelial cell interactions.
The methodology facilitates the study of shear stress-induced changes in both cell types.

Conclusions:

This in vitro model provides a valuable tool for studying endothelial cell and leukocyte behavior under physiologically relevant shear stress conditions.
The system enables deeper insights into vascular biology and the mechanisms of EC dysfunction.
Further research can utilize this methodology to explore various vascular pathologies and therapeutic interventions.