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Relationship between polymorphisms in the CRP, LEP and LEPR genes and high sensitivity C-reactive protein levels in

Pilar Navarro1, Olaya de Dios1, Teresa Gavela-Pérez1

  • 1.

Clinical Chemistry and Laboratory Medicine
|March 29, 2017
PubMed

Insights

Genetic variations in C-reactive protein (CRP), leptin (LEP), and leptin receptor (LEPR) genes are linked to high-sensitivity CRP (hs-CRP) levels in Spanish children. These findings suggest multiple genetic factors influence inflammation markers in pediatric populations.

Area of Science:

  • Genetics
  • Pediatrics
  • Biochemistry

Background:

  • Inflammation markers like high-sensitivity C-reactive protein (hs-CRP) are crucial in pediatric health.
  • Investigating genetic associations with hs-CRP levels can elucidate individual variability in inflammatory responses.
  • Single nucleotide polymorphisms (SNPs) in key genes are potential determinants of hs-CRP levels.

Purpose of the Study:

  • To examine the relationship between SNPs in the C-reactive protein (CRP), leptin (LEP), and leptin receptor (LEPR) genes and hs-CRP levels.
  • To analyze these associations in two independent cohorts of healthy Spanish children across different age groups.
  • To identify specific genetic variants that influence hs-CRP concentrations in children.

Main Methods:

  • hs-CRP levels were quantified using a high-sensitivity C-Reactive Protein ELISA kit in 646 children (6-8 years) and 707 children (12-16 years).
  • Genotyping of four CRP SNPs (rs1205, rs1130864, rs2794521, rs1800947), one LEP SNP (rs7799039), and two LEPR SNPs (rs1137100, rs1137101) was performed using TaqMan® assays.
  • Statistical analyses were conducted to determine significant associations between genotypes, haplotypes, and hs-CRP levels.

Main Results:

  • All four CRP SNPs demonstrated a significant association with hs-CRP levels in both child cohorts (p<0.05).
  • Two CRP haplotypes (ACGA and GCGG) were significantly linked to lower hs-CRP levels at both investigated ages (p<0.05).
  • Polymorphisms in the LEPR (rs1137100, rs1137101) and LEP (rs7799039) genes also showed significant associations with hs-CRP levels in both cohorts (p<0.05).

Conclusions:

  • hs-CRP levels in healthy Spanish children are associated not only with CRP gene polymorphisms but also with LEP and LEPR gene polymorphisms.
  • These findings indicate that genetic variations beyond the CRP gene play a role in determining hs-CRP levels in pediatric populations.
  • The study highlights the complex genetic architecture influencing inflammatory marker levels in children.
Abstract

Keywords:
CRP polymorphismsLEP polymorphismschildrenhs-CRP levels

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