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Related Experiment Video

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An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples
08:58

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Quick and effective method of bone marrow mesenchymal stem cell extraction.

Zivile Gudleviciene1, Gabrielis Kundrotas2, Regina Liudkeviciene3

  • 1National Cancer Institute, Biobank, Santariskiu 1, 08660 Vilnius, Lithuania, tel. +37052190909, fax. +37052720164.

Open Medicine (Warsaw, Poland)
|March 30, 2017
PubMed
Summary

Red blood cell (RBC) lysis enables efficient isolation of mesenchymal stem cells (MSCs) from significantly less bone marrow. This method yields adequate therapeutic MSC doses in a similar timeframe, proving advantageous over density-gradient techniques.

Keywords:
Bone marrowCell therapyFicoll gradientMesenchymal stem cellsRed blood cell lysis

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Area of Science:

  • Stem cell biology
  • Regenerative medicine
  • Cellular therapy

Background:

  • Mesenchymal stem cells (MSCs) are utilized in clinical trials for immune regulation, hematopoiesis, and tissue regeneration.
  • Human bone marrow is the primary source for clinical-grade MSCs.
  • Current methods often require large bone marrow volumes (20-50 ml) for sufficient cell yield.

Purpose of the Study:

  • To evaluate an alternative method for mesenchymal stem cell (MSC) extraction.
  • To compare the efficacy of red blood cell (RBC) lysis versus density gradient for MSC isolation.
  • To determine if a reduced bone marrow volume can yield adequate therapeutic MSC doses.

Main Methods:

  • A pilot study involving 8 healthy bone marrow donors.
  • Evaluation of two MSC extraction techniques: density gradient from 60 ml bone marrow and RBC lysis from 6 ml bone marrow.
  • Comparison of cell yield and isolation time between the two methods.

Main Results:

  • Efficient isolation of human MSCs was achieved using RBC lysis from a 10-fold smaller bone marrow volume (6 ml).
  • Adequate therapeutic doses of MSCs were obtained within a similar timeframe (3-4 weeks) using the RBC lysis method.
  • The RBC lysis method yielded comparable MSC amounts to the standard large-volume density-gradient method.

Conclusions:

  • Mesenchymal stem cell (MSC) isolation via RBC lysis is an effective and advantageous method compared to standard density-gradient isolation.
  • Utilizing RBC lysis allows for the procurement of sufficient MSCs from significantly reduced bone marrow volumes.
  • This optimized method holds promise for more efficient and less invasive MSC procurement for clinical applications.