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Related Experiment Videos

Two-Dimensional Gel Electrophoresis: A Reference Protocol.

Veronica M Saia-Cereda1, Adriano Aquino1, Paul C Guest1

  • 1Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), RuaMonteiroLobato, 25513083-862, Campinas, SP, Brazil.

Advances in Experimental Medicine and Biology
|March 30, 2017
PubMed
Summary
This summary is machine-generated.

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Two-dimensional gel electrophoresis (2DE) is a long-standing proteomic technique. This protocol details its application in analyzing biological samples like blood and brain tissue for disease research.

Area of Science:

  • Proteomics
  • Biochemistry
  • Molecular Biology

Background:

  • Two-dimensional gel electrophoresis (2DE) has been a foundational technique in proteomics for over 40 years.
  • It predates the formal coining of the term 'proteomics' in the early 1990s.
  • 2DE has been instrumental in studying diseases like cancer, diabetes, heart disease, and psychiatric disorders.

Purpose of the Study:

  • To present a general protocol for two-dimensional gel electrophoresis (2DE).
  • To demonstrate the applicability of 2DE in the proteomic analysis of various biological samples.
  • To facilitate the study of disease mechanisms through protein profiling.

Main Methods:

  • The core method described is two-dimensional gel electrophoresis (2DE).
  • The protocol is designed for the analysis of complex biological samples.
Keywords:
2D-DIGE2DE2DE-MSProteomicsTwo-dimensional gel electrophoresis

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  • Specific sample types include blood serum, plasma, and diverse tissue homogenates, such as brain tissue.

    • Main Results:

    • The chapter provides a detailed, generalizable protocol for 2DE.
    • The protocol is validated for its utility across different biological matrices.
    • Successful application in disease-related proteomic studies is implied.

    Conclusions:

    • Two-dimensional gel electrophoresis (2DE) remains a valuable and versatile proteomic tool.
    • The presented protocol offers a standardized approach for analyzing protein expression in various biological samples.
    • This technique continues to contribute significantly to understanding disease pathologies.