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Complement-mediated cytolytic activity associated with cytomegalovirus complement-fixing IgM antibody
1Department of Virology, St. George's Hospital Medical School, University of London, England.
Cytomegalovirus-specific complement-fixing IgM (CMV CF-IgM) antibody was detected in 12/84 (14.3 percent) sera containing CMV-specific IgM. Higher titres were obtained by using a purified antigen preparation containing enveloped virus particles and membrane fragments when compared with a crude extract of infected cells, suggesting that the CF component of CMV IgM reacts predominantly with antigens on the surface of the infected cells. This also accounts for its complement-mediated cytolytic activity. Neither CMV-specific non-complement-fixing IgM nor CMV-specific IgG demonstrates cytolytic activity.
Cytomegalovirus-specific complement-fixing IgM (CMV CF-IgM) antibody was detected in 12/84 (14.3 percent) sera containing CMV-specific IgM. Higher titres were obtained by using a purified antigen preparation containing enveloped virus particles and membrane fragments when compared with a crude extract of infected cells, suggesting that the CF component of CMV IgM reacts predominantly with antigens on the surface of the infected cells. This also accounts for its complement-mediated cytolytic activity. Neither CMV-specific non-complement-fixing IgM nor CMV-specific IgG demonstrates cytolytic activity.