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Günther Gerisch1,2

  • 1Max-Planck-Institut für Biologie (Abt. Weidel) Tübingen, Deutschland.

Wilhelm Roux' Archiv Fur Entwicklungsmechanik Der Organismen
|March 30, 2017
PubMed
Summary

Cellular morphogenetic capacities in Dictyostelium discoideum change after food deprivation. Aggregation peaks at 8-10 hours, while polarization capacity maximizes at 12 hours, influencing aggregation patterns.

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Area of Science:

  • Cellular and developmental biology
  • Microbial morphogenesis
  • Dictyostelium discoideum biology

Context:

  • Cellular behavior and differentiation are significantly influenced by nutrient availability.
  • Understanding the temporal dynamics of cell aggregation and polarization is crucial in developmental biology.
  • Dictyostelium discoideum serves as a model organism for studying multicellular development and cell-cell communication.

Purpose:

  • To determine the precise timing of maximum aggregation capacity in single suspended Dictyostelium discoideum cells.
  • To ascertain the time required for cell aggregates (agglutinates) to exhibit maximal polarization in response to external stimuli.
  • To elucidate the relationship between cellular functional age and morphogenetic capacities post-food exhaustion.

Summary:

  • Following food exhaustion, Dictyostelium discoideum cells exhibit a timed sequence of morphogenetic changes.
  • Maximum aggregation capacity is observed 8-10 hours post-starvation, characterized by metachromatic granule accumulation.
  • Polarization capacity in cell aggregates is dependent on both cell age and aggregate age, peaking around 12 hours post-starvation.

Impact:

  • The 2-hour temporal difference between peak aggregation and polarization capacities can explain observed aggregation patterns, such as centerless formations in synchronized populations and primary center appearance in non-synchronized cultures.
  • This study provides critical temporal data for understanding cell-cell interactions and pattern formation during Dictyostelium development.
  • Findings contribute to the broader understanding of how environmental cues trigger and regulate developmental processes in cellular slime molds.

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