Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antonio Garcia-Bellido1

  • 1Zoologisch-vergleichend anatomischen Institut der Universität Zürich, Schweiz.

Wilhelm Roux' Archiv Fur Entwicklungsmechanik Der Organismen
|March 30, 2017
PubMed
Summary

Male germ cell multiplication in transplanted testes depends on host nutrition, not adult glands. Median Neurosecretory Brain Cells (MNBC) indirectly influence multiplication via protein metabolism.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Wilhelm Roux' Archiv fur Entwicklungsmechanik der Organismen·2017
Same author

Histotypic reaggregation of dissociated imaginal disc cells ofDrosophila melanogaster culturedin vivo.

Wilhelm Roux' Archiv fur Entwicklungsmechanik der Organismen·2017
Same author

Ecdysone induction of puffing activity in salivary glands ofDrosophila melanogaster grown in adult abdomens.

Wilhelm Roux' Archiv fur Entwicklungsmechanik der Organismen·2017
Same author

Developmental analysis of two wing scalloping mutantsct <sup>6</sup> andBx <sup>J</sup> ofDrosophila melanogaster.

Wilhelm Roux's archives of developmental biology·2017
Same author

Extramacrochaetae, a trans-acting gene of the achaete-scute complex of Drosophila involved in cell communication.

Roux's archives of developmental biology : the official organ of the EDBO·2017
Same author

Etxtramacrochaetae, a trans-acting gene of the achaete-scute complex of drosophila involved in cell communication.

Roux's archives of developmental biology : the official organ of the EDBO·2017

Area of Science:

  • Developmental biology
  • Endocrinology
  • Reproductive biology

Context:

  • Investigating the influence of host endocrine glands and nutritional status on the development of implanted testes.
  • Examining the role of Median Neurosecretory Brain Cells (MNBC) and Corpus allatum in regulating germ cell proliferation and differentiation.

Purpose:

  • To elucidate the specific factors within adult hosts that control male germ cell multiplication and differentiation.
  • To determine the endocrine and nutritional requirements for in vivo testis development.

Summary:

  • Testes transplanted into various adult hosts (virgin females, mated females, males) showed similar timing for spermatid differentiation but varied germ cell multiplication rates.
  • Inactivation or implantation of adult host glands revealed that Median Neurosecretory Brain Cells (MNBC), not the Corpus allatum, are crucial for germ cell multiplication.
  • Host nutritional conditions significantly impact germ cell multiplication, with improved outcomes in castrated females or those receiving casein hydrolysate.
  • While MNBCs regulate protein metabolism, germ cell multiplication is ultimately driven by the germ cells' autonomous protein synthesis.

Impact:

  • Identifies MNBCs as key regulators of male germ cell development through their control of host metabolism.
  • Highlights the critical role of host nutrition in supporting germ cell proliferation.
  • Provides insights into the hormonal and nutritional milieu required for in vivo gametogenesis, relevant for reproductive biology research.

Related Experiment Videos