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Anaerobic bacteria in the intestinal microbiota of Brazilian children

Silvia T Talarico1, Florenza E Santos1, Katia Galeão Brandt2

  • 1Universidade de São Paulo, Faculdade de Ciências Farmacêuticas, Departamento de Análises Clínicas e Toxicológicas, São Paulo/SP, Brazil.

Clinics (Sao Paulo, Brazil)
|March 30, 2017
PubMed

Insights

Bifidobacterium dominated the infant gut microbiota in the first year, with Escherichia coli also abundant. Anaerobic bacteria like Faecalibacterium prausnitzii emerged later, influenced by environment and socioeconomic factors.

Area of Science:

  • Microbiology
  • Infant Gut Microbiome Research
  • Neonatal Health

Background:

  • Neonatal gut microbiota development is crucial for infant health.
  • Anaerobic bacteria colonize the neonatal gut environment.
  • Early life microbial colonization influences long-term health outcomes.

Purpose of the Study:

  • To evaluate the colonization patterns of Bifidobacterium, Lactobacillus, and Lactococcus in Brazilian infants during their first year.
  • To correlate these bacterial levels with Escherichia coli abundance.
  • To assess the presence of anaerobic bacteria like Eubacterium limosum and Faecalibacterium prausnitzii.

Main Methods:

  • Collection of fecal samples from 12 Brazilian infants over their first year of life.
  • Real-time PCR detection of 16S rRNA for anaerobic and facultative bacteria.
  • Analysis of Bifidobacterium, Lactobacillus, Lactococcus, Escherichia coli, Eubacterium limosum, and Faecalibacterium prausnitzii presence.

Main Results:

  • Bifidobacterium showed the highest levels throughout the first year.
  • Escherichia coli and Lactobacillus were also consistently detected.
  • Faecalibacterium prausnitzii was primarily detected in later samples, while Eubacterium limosum was rare.

Conclusions:

  • Infant gut microbiota composition aligns with global findings for milk-fed infants.
  • Environmental factors, indicated by high E. coli abundance, influence intestinal colonization.
  • Anaerobic genera were present in Brazilian infants from lower socioeconomic backgrounds, similar to developed countries.
Abstract

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