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Related Experiment Videos

Exploring Human Antimicrobial Antibody Responses on a Single B Cell Level.

Daniel Hofmann1, Jonathan R Lai2

  • 1Evotec AG, Manfred Eigen Campus, Hamburg, Germany daniel.hofmann@evotec.com jon.lai@einstein.yu.edu.

Clinical and Vaccine Immunology : CVI
|March 31, 2017
PubMed
Summary

Single B cell cloning enables the development of human monoclonal antibodies (mAbs) for immunotherapy and vaccine design. This approach reveals pathogen vulnerabilities and guides the creation of effective antibody responses against infections.

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Area of Science:

  • Immunology
  • Microbiology
  • Biotechnology

Background:

  • Monoclonal antibodies (mAbs) derived from single B cell cloning are crucial for understanding host-pathogen interactions.
  • This technique aids in developing antimicrobial immunotherapies and designing vaccines.
  • It provides insights into the human immunoglobulin repertoire's response to pathogens.

Purpose of the Study:

  • To summarize recent advancements in single B cell cloning techniques.
  • To highlight the application of single B cell cloning in developing novel immunotherapies.
  • To underscore its role in structure-based vaccine design.

Main Methods:

  • Single B cell cloning to isolate antibody-producing cells.
  • Sequencing of immunoglobulin variable domains from single B cells.
Keywords:
antibody discoveryimmune responsesimmunotherapysingle B cell sorting

Related Experiment Videos

  • Characterization of the binding and functional activities of derived monoclonal antibodies.

    • Main Results:

    • Identification of pathogen neutralization and opsonization sites.
    • Understanding the evolution of protective antibodies during infection.
    • Generation of fully human monoclonal antibodies for therapeutic use.

    Conclusions:

    • Single B cell cloning is a powerful tool for dissecting antibody responses to pathogens.
    • This method accelerates the development of targeted immunotherapies and vaccines.
    • It offers a roadmap for eliciting protective antibody immunity through rational vaccine design.