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NO-Synthase Activity in Patients with Coronary Heart Disease Associated with Hypertension of Different Age Groups

Anna Besedina1

  • 1Department of Family Medicine, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine.

Journal of Medical Biochemistry
|March 31, 2017
PubMed

Insights

Coronary heart disease (CHD) patients show reduced nitric oxide (NO) production from endothelial nitric oxide synthase (eNOS) and increased NO from inducible nitric oxide synthase (iNOS), especially in older individuals and those with hypertension.

Area of Science:

  • Cardiovascular Research
  • Biochemistry
  • Medical Science

Background:

  • Coronary heart disease (CHD) is a leading global cause of mortality.
  • Hypertension is a significant independent risk factor for CHD development.
  • Dysfunctional nitric oxide (NO) generation is implicated in CHD pathogenesis.

Purpose of the Study:

  • To investigate the activity of endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) in patients with isolated CHD.
  • To compare NOS activity in patients with CHD associated with hypertension (HT) across different age groups.

Main Methods:

  • Enrolled 50 patients with isolated CHD and 42 with CHD and HT.
  • Quantified nitric oxide synthase (NOS) activity by measuring nitrite anion formation.

Main Results:

  • Elderly donors exhibited significantly increased iNOS activity.
  • Patients with isolated CHD showed significantly reduced cNOS activity compared to controls.
  • cNOS activity reduction was more pronounced in elderly CHD patients.
  • eNOS activity alterations were more significant in CHD patients with hypertension.
  • cNOS inhibition correlated with a sharp increase in iNOS activity.

Conclusions:

  • Endothelial dysfunction in CHD with HT involves reduced NO synthesis by cNOS and increased systemic NO from iNOS.
  • The deficit in endothelial NO and excess "harmful" NO from iNOS are exacerbated in elderly patients.
Abstract

Keywords:
NO-synthasecoronary heart diseasehypertensionnitric oxide

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