Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stalling autophagy: a new function for Listeria phospholipases.

Ivan Tattoli1, Matthew T Sorbara2, Dana J Philpott2

  • 1Department of Laboratory Medicine and Pathobiology, University of Toronto, M6G 2T6, Toronto, Canada. ; Department of Immunology, University of Toronto, M6G 2T6, Toronto, Canada.

Microbial Cell (Graz, Austria)
|March 31, 2017
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel <i>DNASE2</i> Variants Linked With Neonatal Acute Liver Failure and Monogenic Systemic Lupus Erythematosus.

The Journal of rheumatology·2026
Same author

NOD2 drives regenerative fetal-like reprogramming in the intestinal epithelium.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Host response to bacteria induces a shift towards the enteroendocrine cell lineage in the murine enteroid model.

PLoS pathogens·2026
Same author

ACSA2 is not astrocyte-specific: implications for cell sorting strategies in the rodent brain.

Frontiers in cellular neuroscience·2026
Same author

Diagnostic Evaluation of Small Intestinal Microbial Overgrowth: A Cross-Sectional Comparison of Glucose and Lactulose Breath Tests.

Journal of clinical medicine·2025
Same author

Acute diverticulitis in 2024: current evidence to prevent onset and recurrence.

Minerva gastroenterology·2025

Listeria monocytogenes phospholipases PI-PLC and PC-PLC aid bacterial escape from host cell autophagy. These toxins inhibit autophagic flux, facilitating intracellular survival and spread.

Area of Science:

  • Bacteriology
  • Cell Biology
  • Infectious Diseases

Background:

  • Listeria monocytogenes invades host cells and escapes vacuoles using listeriolysin O (LLO).
  • L. monocytogenes also secretes PI-PLC and PC-PLC phospholipases, aiding vacuole escape and cell-to-cell spread.
  • The role of these phospholipases during the cytosolic stage of infection was previously uncharacterized.

Purpose of the Study:

  • To investigate the role of Listeria monocytogenes PI-PLC and PC-PLC during the cytosolic stage of infection.
  • To determine if these phospholipases play a role in bacterial escape from host cell autophagy.

Main Methods:

  • The study likely involved bacterial infection models in cell culture.
  • Investigated the interaction of L. monocytogenes phospholipases with the host autophagy pathway.
Keywords:
AutophagyListeriamTOR

Related Experiment Videos

  • Assessed the impact of phospholipase activity on autophagic flux and bacterial survival.

    • Main Results:

    • L. monocytogenes PI-PLC and PC-PLC were found to contribute to bacterial escape from autophagy.
    • These phospholipases were shown to directly inhibit the autophagic flux in infected host cells.
    • This inhibition facilitates bacterial survival and replication within the host cell cytosol.

    Conclusions:

    • Listeria monocytogenes phospholipases PI-PLC and PC-PLC have a novel role in evading host immune responses.
    • By inhibiting autophagic flux, these toxins promote bacterial intracellular survival and pathogenesis.
    • Targeting these phospholipases could represent a potential strategy for treating L. monocytogenes infections.