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Coordinate responses to alkaline pH stress in budding yeast
Albert Serra-Cardona1, David Canadell1, Joaquín Ariño1
1Departament de Bioquímica i Biologia Molecular & Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Bellaterra 08193, Barcelona, Spain.
Microbial Cell (Graz, Austria)
|March 31, 2017
Summary
Budding yeast Saccharomyces cerevisiae activates complex signaling pathways to remodel gene expression under alkaline stress. This response involves nutrient homeostasis and coordinated gene product functions.
Area of Science:
- Microbiology
- Molecular Biology
- Yeast Genetics
Background:
- Alkalinization poses a significant stress to Saccharomyces cerevisiae.
- This stress triggers extensive gene expression remodeling.
- Recent research has elucidated numerous signaling pathways involved in the alkaline response.
Purpose of the Study:
- To summarize the current understanding of yeast response to alkaline conditions.
- To highlight the key signaling pathways and homeostatic mechanisms involved.
- To illustrate the integration of signaling pathways in gene regulation.
Main Methods:
- Review and synthesis of existing scientific literature on yeast alkaline stress response.
- Analysis of gene expression data and signaling pathway interactions.
- Identification of conserved regulatory mechanisms.
Main Results:
- The alkaline response involves conserved pathways like Rim101/PacC, calcineurin, and MAP kinase cascades (Wsc1-Pkc1-Slt2).
- Key kinases such as Snf1 and PKA, and oxidative stress pathways are also implicated.
- Nutrient uptake, including phosphate, iron/copper, and glucose homeostasis, is significantly affected.
Conclusions:
- Diverse signaling pathways converge on gene promoters to orchestrate the alkaline stress response.
- Coordinated gene expression allows for functional coupling of gene products.
- Understanding these integrated networks is crucial for comprehending yeast adaptation to environmental stress.