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Clonal outbreaks of [ Pasteurella] pneumotropica biovar Heyl in two mouse colonies
Sadhana Adhikary1, Magne Bisgaard2, Frederik Dagnæs-Hansen3
11 Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Abstract:
The aim of this study was to document the pathogenic role of biovar Heyl of [ Pasteurella] pneumotropica in mouse colonies. Fifty-three isolates associated with mastitis and orbital, cutaneous and vaginal abscesses as well as isolates from the nose and vagina of healthy mice were investigated. According to phenotypic characteristics and rpoB sequencing, the isolates were identified as [ P.] pneumotropica biovar Heyl. Pulsed-field gel electrophoresis (PFGE) revealed five closely related profiles separated by only one to four fragments. The outbreak strains diverged from epidemiologically unrelated strains with the same rpoB sequence type, as shown by the PFGE profiles. The investigation documented that members of biovar Heyl of [ P.] pneumotropica caused disease outbreaks in mouse colonies since the clonality indicated a primary role of [ P.] pneumotropica biovar Heyl in the infections observed.
Insights
Pasteurella pneumotropica biovar Heyl caused disease outbreaks in mouse colonies. Pulsed-field gel electrophoresis (PFGE) confirmed clonality, indicating this biovar
Area of Science:
- Microbiology
- Animal Health
- Infectious Diseases
Background:
- Pasteurella pneumotropica is a common commensal in rodents.
- Biovar Heyl has been implicated in various infections.
- Understanding its pathogenic role is crucial for animal colony health.
Purpose of the Study:
- To investigate the pathogenic role of Pasteurella pneumotropica biovar Heyl in mouse colonies.
- To identify the genetic relatedness of isolates causing disease outbreaks.
- To confirm the causative agent of mastitis and abscesses.
Main Methods:
- Phenotypic characterization of isolates.
- rpoB gene sequencing for identification.
- Pulsed-field gel electrophoresis (PFGE) for strain typing.
Main Results:
- Fifty-three isolates were identified as Pasteurella pneumotropica biovar Heyl.
- PFGE revealed five closely related profiles, indicating clonality.
- Outbreak strains were genetically linked, differing from unrelated strains.
Conclusions:
- Pasteurella pneumotropica biovar Heyl is a significant cause of disease outbreaks in mouse colonies.
- Clonality of isolates supports the primary role of this biovar in observed infections.
- Effective control measures are needed to prevent future outbreaks.