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A flow-cytometric method to evaluate eosinophil-mediated uptake of probiotic Lactobacillus reuteri
Laura S Kraemer1, Todd A Brenner1, Julia O Krumholz1
1Inflammation Immunobiology Section, Laboratory of Allergic Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892, United States.
Journal of Microbiological Methods
|April 1, 2017
Abstract:
Eosinophils are resident leukocytes of gut mucosa. Here we present a combined flow cytometric-antibiotic protection assay to identify mouse eosinophils capable of bacterial uptake, specifically, Gram-positive Lactobacillus reuteri, in studies performed ex vivo. The assay may be adapted for use in vivo.