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Related Experiment Videos

Origin of α-mannosidase activity in CSF.

Anna Tasegian1, Silvia Paciotti1, Maria Rachele Ceccarini1

  • 1Department of Pharmaceutical Sciences, University of Perugia, Perugia, Italy.

The International Journal of Biochemistry & Cell Biology
|April 1, 2017
PubMed
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This summary is machine-generated.

Cerebrospinal fluid (CSF) alpha-mannosidase activity originates from the brain, not plasma. This lysosomal enzyme profile in CSF may indicate neurodegenerative disorders like Parkinson's disease.

Area of Science:

  • Biochemistry
  • Neuroscience
  • Enzymology

Background:

  • Alpha-mannosidase is a key enzyme involved in glycoprotein metabolism.
  • Its activity in cerebrospinal fluid (CSF) is not fully understood, particularly its origin.
  • Investigating CSF alpha-mannosidase may offer insights into neurological conditions.

Purpose of the Study:

  • To determine the origin of alpha-mannosidase activity in human CSF.
  • To compare the isoenzyme profiles of alpha-mannosidase in the frontal gyrus, CSF, and plasma.
  • To assess the potential of CSF alpha-mannosidase as a biomarker for neurodegenerative diseases.

Main Methods:

  • DEAE-cellulose chromatography was employed to analyze alpha-mannosidase activity.
  • Samples analyzed included human frontal gyrus, CSF, and plasma.
Keywords:
BrainCSFLysosomesMAN2B1Parkinsonα-Mannosidase

Related Experiment Videos

  • Isoenzyme profiles were characterized based on pH optima.

    • Main Results:

    • CSF alpha-mannosidase isoenzyme profiles closely resembled those found in the frontal gyrus.
    • Human plasma exhibited a distinct alpha-mannosidase profile, dominated by an intermediate form (pH optimum 5.5).
    • Lysosomal alpha-mannosidase forms A and B (pH optimum 4.5) were present in both frontal gyrus and CSF.

    Conclusions:

    • The intermediate form of alpha-mannosidase in plasma does not appear to cross the blood-brain barrier.
    • Alpha-mannosidase activity in CSF is of lysosomal and brain origin.
    • CSF alpha-mannosidase levels may serve as a biomarker reflecting brain pathology in neurodegenerative disorders, including Parkinson's disease.