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Lactic acidosis: an experimental model.

A I Arieff, A Kerian

Metabolism: Clinical and Experimental

|March 1, 1976

Summary

Diabetic rabbits developed spontaneous lactic acidosis after beta-hydroxybutyric acid and sodium bicarbonate infusions. This model shows altered NAD/NADH ratios and increased lactate, highlighting metabolic disturbances in diabetes.

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Area of Science:

Biochemistry
Physiology
Endocrinology

Background:

Diabetic ketoacidosis is a serious complication of diabetes mellitus.
Lactic acidosis can occur in diabetes, but its spontaneous development is not fully understood.
Alloxan-induced diabetes in rabbits provides a model for studying metabolic derangements.

Purpose of the Study:

To develop a reproducible model of spontaneous lactic acidosis in diabetic rabbits.
To investigate the metabolic changes associated with induced lactic acidosis in a diabetic model.
To compare the response of diabetic versus normal rabbits to the induction protocol.

Main Methods:

Alloxan was used to induce diabetes in rabbits.
Intravenous infusion of beta-hydroxybutyric acid and sodium bicarbonate was administered.
Arterial blood gases, lactate/pyruvate ratio, and NAD/NADH ratio were measured.
Cardiorespiratory parameters were monitored.

Main Results:

Diabetic rabbits developed significant lactic acidosis, with elevated lactate/pyruvate ratios and decreased arterial pH.
The NAD/NADH ratio was significantly lower in rabbits with lactic acidosis compared to normal controls.
Normal rabbits did not develop lactic acidosis under the same experimental conditions.
Continued sodium bicarbonate infusion in diabetic rabbits led to increased blood lactate and cardiorespiratory arrest in 36% of animals.

Conclusions:

A model for spontaneous lactic acidosis was successfully established in alloxan-induced diabetic rabbits.
The model demonstrates altered redox state (NAD/NADH ratio) contributing to lactic acidosis in diabetes.
This experimental model is valuable for studying the pathophysiology and potential treatments of lactic acidosis in diabetes.

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