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Related Experiment Videos

Genetically encoded fluorescent tags.

Kurt Thorn1

  • 1Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158 kurt.thorn@gmail.com.

Molecular Biology of the Cell
|April 1, 2017
PubMed
Summary

Genetically encoded fluorescent tags enable researchers to visualize proteins in live cells using microscopy and quantify them with flow cytometry. This review introduces various tags and guides their selection for diverse biological imaging applications.

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Area of Science:

  • Cell Biology
  • Biotechnology
  • Molecular Imaging

Background:

  • Genetically encoded fluorescent tags are crucial tools in modern cell biology.
  • They allow for real-time visualization and quantification of proteins within live cells and organisms.
  • Revolutionized imaging capabilities with high spatial and temporal resolution.

Purpose of the Study:

  • To provide an introduction to available genetic tags for protein labeling.
  • To discuss the optical and biological properties of different fluorescent tags.
  • To offer guidelines for selecting appropriate tags for experimental needs.

Main Methods:

  • Review of intrinsically fluorescent proteins and those requiring exogenous fluorophores.
  • Discussion of tag properties including spectral characteristics and photostability.
  • Exploration of applications in light microscopy and flow cytometry.

Main Results:

  • Detailed overview of diverse fluorescent protein tags and their functionalities.
  • Comparison of tag performance based on optical and biological parameters.
  • Guidelines for optimal tag selection considering experimental context.

Conclusions:

  • Genetic fluorescent tags are indispensable for advancing cell biology research.
  • Proper tag selection is key to successful protein imaging and quantification.
  • Emerging tools for nucleic acid tagging and biomolecule detection expand research possibilities.

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