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Updated: Aug 15, 2026
12:06
Surface Spreading and Immunostaining of Yeast Chromosomes
Published on: August 9, 2015
The stability of chromosomes in yeast
V L Larionov1, T S Karpova, G A Zhouravleva
1Institute of Cytology, Academy of Sciences of the USSR, Leningrad.
Current Genetics
|January 1, 1987
Summary
UV-induced chromosome instability mutants were identified. Two distinct groups emerged: one with increased recombination and another (CL4, CL8) with unstable chromosome and minichromosome maintenance linked to new genes.
Area of Science:
- Genetics
- Molecular Biology
- Yeast Genetics
Background:
- Chromosome instability is a hallmark of many diseases, including cancer.
- Understanding the genetic basis of chromosome instability is crucial for developing therapeutic strategies.
Purpose of the Study:
- To investigate the genetic factors underlying chromosome instability in yeast.
- To characterize novel genes involved in mitotic chromosome transmission.
Main Methods:
- UV irradiation of haploid yeast strains disomic for chromosome III.
- Analysis of spontaneous inter- and intragenic recombination frequencies.
- Assessment of artificial minichromosome stability.
- Genetic mapping of instability loci.
Main Results:
- Two classes of CL mutants were identified based on recombination levels.
- Mutants CL4 and CL8 exhibited chromosome III instability without elevated recombination.
- CL4 and CL8 mutants showed unstable maintenance of artificial minichromosomes.
- Instability in CL4 and CL8 was attributed to two nonallelic genes, ch14 and ch18.
Conclusions:
- The study identified two novel genes, ch14 and ch18, involved in mitotic chromosome transmission.
- These genes play a role in maintaining chromosome stability, independent of recombination frequency.
- Findings provide insights into the complex mechanisms governing chromosome segregation.
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