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Related Experiment Videos

Multiaxial Polarity Determines Individual Cellular and Nuclear Chirality.

Michael J Raymond1, Poulomi Ray2, Gurleen Kaur3

  • 1Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy NY 12180.

Cellular and Molecular Bioengineering
|April 1, 2017
PubMed
Summary

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Cell chirality, crucial for embryonic development, can now be quantified. This study reveals how cell and nuclear polarization coordinate to establish left-right asymmetry, offering insights into chiral morphogenesis.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Biophysics

Background:

  • Intrinsic cell chirality is vital for establishing left-right (LR) asymmetry during embryonic development.
  • Defects in cell chirality can result in severe birth defects related to laterality.
  • Previous methods for detecting cell chirality relied on in vitro micropatterning systems.

Purpose of the Study:

  • To quantify cell chirality by analyzing the coordination of multiaxial polarization in individual cells and nuclei.
  • To characterize cell chirality in multicellular epithelial patterns using shape polarization and nuclear positioning.
  • To investigate the relationship between cell alignment, nuclear positioning, and directional cell migration.

Main Methods:

  • Utilized an object labeling, connected component-based method for quantitative analysis.
Keywords:
Cell ChiralityCell MorphologyCell PolarityNuclear Morphology

Related Experiment Videos

  • Characterized cell and nuclear shape polarization.
  • Analyzed nuclear positioning within multicellular epithelial cell patterns.

    • Main Results:

    • Quantified cell chirality as the coordination of multiaxial polarization of cells and nuclei.
    • Observed that cells adopt a LR bias at boundaries, with the sharp end facing the leading edge and nuclei positioned at the rear.
    • Found that nuclear positioning exhibits opposite chirality to cell alignment due to rearward positioning.

    Conclusions:

    • Demonstrated a novel method to quantify cell chirality based on multiaxial polarization.
    • Revealed that chiral morphogenesis involves the coordinated polarization of cells and subcellular components like nuclei.
    • Provided deep insights into the cellular and subcellular mechanisms underlying chiral morphogenesis and LR asymmetry.