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Encapsulation of a compartmentalized cytoplasm mimic within a lipid membrane by microfluidics
Marta Sobrinos-Sanguino1, Silvia Zorrilla, Christine D Keating
1Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), 28040, Madrid, Spain. silvia@cib.csic.es monterroso@cib.csic.es grivas@cib.csic.es.
Chemical Communications (Cambridge, England)
|April 1, 2017
Abstract:
There is growing interest in analyzing the effect of microenvironments, which may be mimicked through liquid-liquid phase separation (LLPS), on the reactivity of biological macromolecules. We report the encapsulation by microfluidics of the division protein FtsZ and a LLPS system inside microdroplets and their conversion into permeable vesicles (allowing ligand uptake), with higher yield, homogeneity and biomolecular compatibility than those previously described.