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Formation of Stomach Tissue by Organoid Culture Using Mouse Embryonic Stem Cells
Taka-Aki K Noguchi1, Akira Kurisaki2,3
1Graduate School of Life and Environmental Sciences, The University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki, Japan. taka.noguchi@aist.go.jp.
Methods in Molecular Biology (Clifton, N.J.)
|April 1, 2017
Abstract:
In this chapter, we describe a method for the induction of stomach organoids from mouse embryonic stem (ES) cells. We used an embryoid body-based differentiation method to induce gastric primordial epithelium covered with mesenchyme and further differentiate it in Matrigel by 3D culture. The differentiated organoid contains both corpus- and antrum-specific mature gastric tissue cells. This protocol may be useful for a variety of studies in developmental biology and disease modeling of the stomach.