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Updated: Aug 19, 2026

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
Published on: December 7, 2016
Detection of the Unstable Hb Köln (HBB: c.295G>A) by a Capillary Electrophoresis Method
You-Qiong Li1, Li-Hua Ye2, Yun Mo3
1a The Center of Reproduction and Genetic , People's Hospital of Guangxi Zhuang Autonomous Region , Nanning , Guangxi , People's Republic of China.
Abstract:
Hb Köln (HBB: c.295G>A) is an unstable β-globin gene variant with a GTG>ATG substitution at codon 98. This variant is quite frequent in Europe and the USA but rare in China. It can easily be misdiagnosed as Hb Constant Spring (Hb CS; HBA2: c.427T>C) by high performance liquid chromatography (HPLC), but detection and quantification of both Hb Köln and degraded Hb Köln by capillary electrophoresis (CE) are possible. Thus, we concluded that CE was the preferred method for Hb Köln detection.
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