Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Replication02:40

DNA Replication

60.0K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
60.0K
The DNA Replication Fork01:02

The DNA Replication Fork

41.1K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
41.1K
The DNA Replication Fork01:02

The DNA Replication Fork

18.5K
No description available
18.5K
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

5.7K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
5.7K
Chromosome Replication02:31

Chromosome Replication

10.7K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
10.7K
Replication in Prokaryotes02:35

Replication in Prokaryotes

98.9K
Overview
98.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-uniform growth probability of Mycobacterium tuberculosis across inoculum densities partially restored by culture filtrate: Implications for supplemented growth assays.

Journal of microbiological methods·2026
Same author

Guanilated Quinolones with Dual Antitubercular and Anti-Inflammatory Activities.

ChemMedChem·2026
Same author

Beyond Inhibition: Sublethal Rifampicin-Induced Molecular Adaptations Confer Phenotypic Drug Tolerance in Mycobacteria.

ACS infectious diseases·2026
Same author

Novel, Achiral 4‑Nitroimidazole Compounds with Potent Antitubercular Activity.

ACS omega·2026
Same author

Identification of determinants of high-fidelity DNA synthesis in Mycobacterium smegmatis DnaE1 through in silico and in vivo approaches.

Nucleic acids research·2025
Same author

Cobamide metabolism, regulation, and adaptation in <i>Mycobacterium tuberculosis</i>.

Journal of bacteriology·2025
Same journal

Structures of the endophytic microbiota during heart rot development in <i>Abies georgei</i> var. <i>smithii</i>.

Microbiology spectrum·2026
Same journal

High <i>Yersinia</i> prevalence in tonsils of wild boars hunted in Northeast Germany using a novel protocol including long cold enrichment.

Microbiology spectrum·2026
Same journal

Genetic variation, recombinant characteristics, and seroprevalence analysis of echovirus 3 causing severe and mild cases of hand, foot, and mouth disease in Guizhou Province.

Microbiology spectrum·2026
Same journal

Mycelial morphology influenced aerobic DNRA in <i>Streptomyces mediolani</i> EM-B2: short rod-shaped mycelium showed markedly greater efficiency than long filamentous mycelium.

Microbiology spectrum·2026
Same journal

Performance and practicality of 16S nanopore sequencing for routine bacterial identification in clinical samples.

Microbiology spectrum·2026
Same journal

Molecular characterization and correlation with β-lactam resistance of penicillin-binding protein2x, 2b, and 1a of <i>Streptococcus pneumoniae</i> in clinical pneumococcal isolates.

Microbiology spectrum·2026
See all related articles

Related Experiment Video

Updated: Feb 10, 2026

Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis
08:48

Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis

Published on: July 11, 2025

634

DNA Replication in Mycobacterium tuberculosis.

Zanele Ditse1, Meindert H Lamers2, Digby F Warner3,4

  • 1Centre for HIV and STIs, National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, 2131, South Africa.

Microbiology Spectrum
|April 1, 2017
PubMed
Summary
This summary is machine-generated.

Mycobacterium tuberculosis genome maintenance is crucial for survival and adaptation. This study explores DNA replication and repair mechanisms, highlighting how mutations drive drug resistance in tuberculosis.

More Related Videos

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
10:06

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

Published on: February 26, 2018

7.8K
Growth of Mycobacterium tuberculosis Biofilms
09:03

Growth of Mycobacterium tuberculosis Biofilms

Published on: February 15, 2012

24.4K

Related Experiment Videos

Last Updated: Feb 10, 2026

Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis
08:48

Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis

Published on: July 11, 2025

634
Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
10:06

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

Published on: February 26, 2018

7.8K
Growth of Mycobacterium tuberculosis Biofilms
09:03

Growth of Mycobacterium tuberculosis Biofilms

Published on: February 15, 2012

24.4K

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Genome replication and maintenance are vital for organism survival and propagation.
  • Mycobacterium tuberculosis (M. tuberculosis), an obligate pathogen, faces significant host stresses during infection.
  • Chromosomal mutations are known to facilitate M. tuberculosis adaptation to these stresses, evidenced by drug resistance and genetic diversity.

Purpose of the Study:

  • To summarize current understanding of DNA replication machinery in M. tuberculosis.
  • To discuss the role of mycobacterial DNA polymerases in mutagenesis.
  • To explore the potential contribution of DNA replication to M. tuberculosis stress tolerance and the emergence of drug resistance.

Main Methods:

  • Comparative genomic analyses.
  • Review of existing literature on M. tuberculosis DNA replication and repair.
  • Discussion of mycobacterial DNA polymerase functions.

Main Results:

  • M. tuberculosis possesses an expanded complement of DNA polymerases, suggesting a significant role in mutagenesis.
  • DNA replication regulation and coordination with cell division may contribute to stress tolerance.
  • Mutagenic mechanisms driving M. tuberculosis evolution and adaptation remain incompletely understood.

Conclusions:

  • Understanding M. tuberculosis DNA replication is key to deciphering its adaptation and evolution.
  • The expanded DNA polymerase repertoire likely contributes to mutagenesis and adaptation under stress.
  • Further research into DNA replication mechanisms could reveal targets for combating tuberculosis drug resistance.