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A Customizable Chamber for Measuring Cell Migration.

Aniqa N Chowdhury1, Huu Tri Vo1, Sharon Olang1

  • 1Department of Bioengineering, Clemson University.

Journal of Visualized Experiments : Jove
|April 1, 2017
PubMed
Summary
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This study presents an easy method for creating cell migration chambers, enabling customizable chemical gradients for observing cell movement. This technique is accessible even for undergraduate students, simplifying complex cell migration assays.

Area of Science:

  • Cell Biology
  • Biotechnology
  • Biomaterials

Background:

  • Cell migration is crucial for immune responses, growth, and wound healing.
  • Traditional cell migration assays, like Boyden chambers, lack geometric flexibility.
  • Existing microfluidic methods can be complex to implement.

Purpose of the Study:

  • To develop an accessible method for creating cell culture chambers for studying cell migration.
  • To enable the generation of customizable linear chemical concentration gradients.
  • To provide a versatile platform for various cell migration research applications.

Main Methods:

  • Fabrication of a microchannel chamber using an acrylic insert and poly(dimethylsiloxane) (PDMS).
  • Creation of linear chemical gradients using an agarose block soaked in soluble agents.

Related Experiment Videos

  • Quantification of cell migration via time-lapse imaging of the microchannel chamber.

    • Main Results:

    • Successfully created customizable cell migration chambers with varying well shapes and sizes.
    • Demonstrated the ability to generate linear concentration gradients of soluble factors.
    • Developed a user-friendly assay suitable for undergraduate research.

    Conclusions:

    • The described method offers an easy-to-use and customizable approach to studying cell migration.
    • This technique overcomes limitations of traditional assays and complexity of existing microfluidic devices.
    • The customizable nature of the chamber allows for diverse applications in cell migration research.