Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sensitizing pathogens to antibiotics using the CRISPR-Cas system.

Moran Goren1, Ido Yosef1, Udi Qimron1

  • 1Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel.

Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy
|April 2, 2017
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering Phages to Fight Multidrug-Resistant Bacteria.

Chemical reviews·2024
Same author

Gamma-Mobile-Trio systems are mobile elements rich in bacterial defensive and offensive tools.

Nature microbiology·2024
Same author

Systematic interrogation of CRISPR antimicrobials in Klebsiella pneumoniae reveals nuclease-, guide- and strain-dependent features influencing antimicrobial activity.

Nucleic acids research·2024
Same author

Contracting the Host Range of Bacteriophage T7 Using a Continuous Evolution System.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Inhibition of host cell division by T5 protein 008 (Hdi).

Microbiology spectrum·2023
Same author

An efficient, scarless, selection-free technology for phage engineering.

RNA biology·2023

The clustered regularly interspaced short palindromic repeats-CRISPR associated (CRISPR-Cas) system offers a novel approach to combat antibiotic-resistant pathogens by editing bacterial populations and eliminating resistance genes. This technology may significantly reduce the threat of drug-resistant bacteria.

Area of Science:

  • Microbiology
  • Genetics
  • Biotechnology

Background:

  • Antibiotic resistance is a growing global health threat driven by extensive antibiotic use.
  • Existing strategies include new antibiotics, antimicrobial peptides, and bacteriophages.
  • The clustered regularly interspaced short palindromic repeats-CRISPR associated (CRISPR-Cas) system is a novel prokaryotic defense mechanism.

Purpose of the Study:

  • To review studies utilizing the CRISPR-Cas system against antibiotic-resistant pathogens.
  • To explore CRISPR-Cas applications in editing bacterial populations and eliminating resistance genes.
  • To assess the potential of CRISPR-Cas in creating selection pressure for antibiotic-sensitive bacteria.

Main Methods:

  • Review of pioneering studies on CRISPR-Cas system applications in bacterial manipulation.
Keywords:
Antibiotic resistanceBacteriophage delivery vectorsESKAPE pathogensSelective pressure

Related Experiment Videos

  • Analysis of strategies for specific DNA cleavage and gene editing in pathogens.
  • Examination of methods for delivering CRISPR-Cas tools into bacterial populations.

    • Main Results:

    • The CRISPR-Cas system can be guided to cleave specific DNA sequences in pathogens.
    • Studies demonstrate the elimination of antibiotic resistance genes using CRISPR-Cas.
    • Combining population editing and gene elimination creates artificial selection for antibiotic sensitivity.

    Conclusions:

    • The CRISPR-Cas system presents a sophisticated tool for combating drug-resistant pathogens.
    • Intelligent design and efficient delivery are crucial for maximizing CRISPR-Cas efficacy.
    • This technology holds significant potential to reduce the threat of antibiotic resistance.