Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Facilitating trypanosome imaging.

Marius Glogger1, Ines Subota1, Anna Pezzarossa2

  • 1Department of Cell and Developmental Biology, Biocenter, University of Würzburg, Würzburg, Germany.

Experimental Parasitology
|April 2, 2017
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell surface localisation of GPI-anchored receptors in <i>Trypanosoma brucei</i>.

eLife·2026
Same author

RNA decay sharpens antigenic variation in trypanosomes.

Trends in parasitology·2026
Same author

Organelle mechanics: A brace for the Plasmodium basal body.

Current biology : CB·2026
Same author

Robust HLA-B-restricted CD8+ T cell responses in chronic HBV infection.

JHEP reports : innovation in hepatology·2026
Same author

Insights from aquaporin structures into drug-resistant sleeping sickness.

eLife·2026
Same author

The Dual Role of the 16mer Motif Within the 3' Untranslated Region of the Variant Surface Glycoprotein of Trypanosoma brucei.

Molecular microbiology·2025
Same journal

Exploring polyporphyrin isolated from Carica papaya seed oil in the control of Anopheles gambiae (Diptera: Culicidae) () mosquito.

Experimental parasitology·2026
Same journal

Trypanocidal activity of nanoemulsion loaded with triterpene 3β,6β,16β-trihydroxylup-20(29)-ene from Combretum leprosum against Trypanosoma cruzi Y strain.

Experimental parasitology·2026
Same journal

Nanotechnology in the management of neglected protozoan diseases: Current advances, translational challenges, and future prospects.

Experimental parasitology·2026
Same journal

Corrigendum to "Expression profiling of GP63 isoforms with predicted nuclear localization signals in Leishmania (Viannia) braziliensis" [Experimental Parasitology 286 (2026) 109155].

Experimental parasitology·2026
Same journal

Identification of ZINC database-derived antitrypanosomal candidates targeting Trypanosoma congolense lysosomal-α-mannosidase using computational, biochemical and in vivo methods.

Experimental parasitology·2026
Same journal

Expression profiling of GP63 isoforms with predicted nuclear localization signals in Leishmania (Viannia) braziliensis.

Experimental parasitology·2026
See all related articles

Researchers developed a novel hydrogel to immobilize live trypanosomes, enabling super-resolution microscopy of their cell membranes. This technique revealed distinct membrane domains, offering new insights into trypanosome cell biology.

Area of Science:

  • Cell Biology
  • Biophysics
  • Microscopy

Background:

  • Trypanosomes are crucial model organisms for understanding fundamental cellular processes.
  • Advances in super-resolution microscopy allow visualization of nanoscale biological structures in living cells.
  • Cell motility in trypanosomes has historically hindered high-resolution live-cell imaging.

Purpose of the Study:

  • To develop a method for immobilizing live trypanosomes for high-resolution imaging.
  • To investigate the nanoscale organization of the inner plasma membrane leaflet in live trypanosomes.
  • To explore the potential association of membrane structures with the cytoskeleton.

Main Methods:

  • Fabrication of a poly(ethylene glycol)-based hydrogel using UV-induced thiol-ene crosslinking.
Keywords:
HydrogelMembraneSingle-molecule fluorescence microscopyTrypanosomes

Related Experiment Videos

  • Embedding and culturing of live trypanosomes within the hydrogel for up to one hour at 24°C.
  • Application of super-resolution microscopy with lipid-anchored eYFP to image the inner plasma membrane leaflet.

    • Main Results:

    • The hydrogel effectively immobilizes trypanosomes on the nanometer scale with low autofluorescence and maintains cell viability.
    • Super-resolution imaging revealed non-homogenous distribution of eYFP, indicating specific membrane domains.
    • Ripley's analysis quantified the size of accumulated domains (r=170±5 nm) and dilute domains (r>115±15 nm).

    Conclusions:

    • The developed hydrogel is a viable tool for high-resolution imaging of motile cells like trypanosomes.
    • Live trypanosome plasma membranes exhibit nanoscale domain structuring.
    • This membrane organization is hypothesized to be linked to the underlying cytoskeleton.