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In Vivo and Explant Electroporation of Morpholinos in the Developing Mouse Retina
Devi Krishna Priya Karunakaran1, Rahul Kanadia2
1Department of Physiology and Neurobiology, University of Connecticut, 75 North Eagleville Road, Storrs, CT, 06269, USA.
Methods in Molecular Biology (Clifton, N.J.)
|April 2, 2017
Abstract:
Neonatal in vivo electroporations and retinal explant electroporations have been widely employed in understanding the effects of loss or gain of function of protein-coding genes in retinal development. Here, we describe a rapid and efficient delivery of morpholinos to add another tool to perturb gene expression during mouse retinal development.