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System-level perturbations of cell metabolism using CRISPR/Cas9
Tadas Jakočiūnas1, Michael K Jensen1, Jay D Keasling2
1The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Denmark.
Current Opinion in Biotechnology
|April 3, 2017
Summary
CRISPR/Cas9 gene editing tools offer advanced, cost-effective genome engineering for metabolic engineering. These methods enable precise pathway modifications and transcriptional regulations for systems-level studies.
Area of Science:
- Molecular Biology
- Biotechnology
- Systems Biology
Background:
- CRISPR/Cas9 (clustered regularly interspaced palindromic repeats and the associated protein Cas9) techniques have revolutionized genome engineering.
- These tools provide cost-effective and advanced methods for transcriptional reprogramming studies.
Purpose of the Study:
- To review recent advancements in CRISPR/Cas9 applications for metabolic engineering.
- To highlight the use of CRISPR-based tools for systems-level perturbations and model-guided genome editing.
Main Methods:
- Review of recent literature on CRISPR/Cas9 applications in metabolic engineering.
- Focus on studies employing CRISPR for single and multiplex pathway modifications.
- Emphasis on transcriptional regulations and genome-wide perturbations.
Main Results:
- CRISPR/Cas9 enables sophisticated genome engineering and transcriptional reprogramming.
- These tools facilitate efficient single and multiplex pathway modifications.
- CRISPR-based approaches are effective for systems-level perturbations and model-guided strategies.
Conclusions:
- CRISPR/Cas9 techniques are pivotal for advancing metabolic engineering and systems biology.
- The versatility of CRISPR tools allows for complex genome editing and regulatory studies.
- Recent studies demonstrate the power of CRISPR in model-guided metabolic engineering.