Porous membrane protected micro-solid-phase extraction: A review of features, advancements and applications

Membrane protected micro-solid-phase extraction (μ-SPE) was introduced in 2006 as an alternative to multistep SPE. μ-SPE is based on packing of very small amount of sorbent inside the porous membrane sheet whose edges are heat sealed to fabricate a μ-SPE device. This device performs clean up, extraction, and pre-concentration in a single step. It suits best for extraction of complex samples as sorbent is effectively protected inside the membrane and extraneous matter cannot adsorb over it. This review summarizes most important aspects of μ-SPE including basic principles, extraction procedure, different formats, sorbents employed and affecting parameters. The article also provides a brief account on modified μ-SPE procedures where μ-SPE was either combined with other techniques or some major changes were introduced in original procedure. Finally, the applications of μ-SPE in environmental, food and biological analysis are described. At the end, advantages and pitfalls of μ-SPE are critically appraised.