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Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus
C L Keen1, S Mendelovitz, G Cohen
1Department of Microbiology, University of Alberta, Edmonton, Canada.
Molecular & General Genetics : MGG
|April 1, 1988
Abstract:
A linear DNA plasmid (pSCL) has been isolated from Streptomyces clavuligerus by a method employing high concentrations of protease. Rate-zonal sedimentation on sucrose gradients was used to purify the plasmid. The plasmid is 12 kb in length and appears to be linked to protein at its 5' termini. A restriction endonuclease map of the plasmid for ten enzymes has been determined. Evidence for terminally repeated sequences is provided by cross-hybridization analysis.