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Variation of virulence and other properties among Sendai virus strains
1Department of Veterinary Public Health, Institute of Public Health, Tokyo.
Abstract:
The virulence of five Sendai virus strains (MN, Z, KN, Mol, and Hm) isolated from laboratory rodents was compared, using 3-week-old female Jcl-ICR mice. The virulence of the strains was Mol, MN, KN, Z, and Hm in decreasing order. The 50% lethal dose and 50% lung consolidation inducing dose of the highest virulent strain differed by the order of more than 10(3) and 10(6), respectively, from those of the lowest virulent one. Other properties such as the growth rate in LLC-MK2 cells, neuraminidase activities, and molecular weights of structural proteins also differed among the virus strains. These results indicate that Sendai virus prevailing in laboratory rodents is not homogenous with respect to virulence and some other properties.
Insights
Sendai virus strains from lab rodents show varied virulence. Differences in lethal dose and lung consolidation were observed, highlighting genetic diversity in these common viruses.
Area of Science:
- Virology
- Immunology
- Rodent Health
Background:
- Sendai virus is a common paramyxovirus affecting laboratory rodents.
- Variations in Sendai virus strains can impact research reproducibility.
- Understanding strain diversity is crucial for animal model integrity.
Purpose of the Study:
- To compare the virulence of five distinct Sendai virus strains.
- To investigate other biological properties of these strains.
- To assess the homogeneity of Sendai virus in laboratory rodent populations.
Main Methods:
- Comparative analysis of five Sendai virus strains (Mol, MN, KN, Z, Hm).
- Virulence assessment in 3-week-old female Jcl-ICR mice.
- Determination of 50% lethal dose (LD50) and 50% lung consolidation dose (LCD50).
- Evaluation of growth kinetics in LLC-MK2 cells and neuraminidase activity.
Main Results:
- Virulence varied significantly among strains, ordered as Mol > MN > KN > Z > Hm.
- The highest virulent strain showed LD50 and LCD50 values over 10^3 and 10^6 times greater than the lowest virulent strain, respectively.
- Differences were also noted in growth rates, neuraminidase activity, and structural protein molecular weights.
Conclusions:
- Sendai virus strains circulating in laboratory rodents are not uniform.
- Significant heterogeneity exists in virulence and other biological characteristics.
- This diversity has implications for experimental outcomes and animal health management.