Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bull Fertility and Its Relation with Density Gradient Selected Sperm.

Lynda Allouche1, Toufik Madani2, Mohamed Mechmeche3

  • 1Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, Ferhat Abbas Setif 1 University, Setif, Algeria.

International Journal of Fertility & Sterility
|April 4, 2017
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel insights into the bovine polled phenotype and horn ontogenesis in Bovidae.

PloS one·2013
Same author

A 3.7 Mb deletion encompassing ZEB2 causes a novel polled and multisystemic syndrome in the progeny of a somatic mosaic bull.

PloS one·2012
Same author

PTGS2-related PGE2 affects oocyte MAPK phosphorylation and meiosis progression in cattle: late effects on early embryonic development.

Biology of reproduction·2011
Same author

Chronic effects of low lead levels on sperm quality, gonadotropins and testosterone in albino rats.

Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie·2009
Same author

Expression of genes involved in prostaglandin E2 and progesterone production in bovine cumulus-oocyte complexes during in vitro maturation and fertilization.

Reproduction (Cambridge, England)·2008

Sperm selection methods impact fertility. High motility and abaxial implantation in selected sperm negatively affect in vivo fertility, while a maximal limit of 47% immotile sperm is optimal.

Area of Science:

  • Reproductive Biology
  • Veterinary Science
  • Animal Breeding

Background:

  • Sperm selection is crucial for assisted reproduction.
  • Limited research exists on the correlation between in vivo fertility and semen parameters post-selection.
  • This study investigates semen parameters after sperm selection to predict fertility.

Purpose of the Study:

  • To assess semen parameters after sperm selection using density gradient separation.
  • To predict in vivo fertility based on these semen parameters.
  • To establish the relationship between semen quality and fertility rates in bulls.

Main Methods:

  • Frozen semen from Montbeliarde bulls was analyzed post-thaw (PT) and post-sperm selection (SSp).
  • Semen parameters evaluated included concentration, motility, morphology, viability, and membrane integrity.
Keywords:
BullFertilityFrozen Semen

Related Experiment Videos

  • Fertility was measured using non-return rates (NRR) and corrected NRR (CNRR), analyzed with Kruskal-Wallis, Chi-square, and Pearson correlation tests.

    • Main Results:

    • Most bulls showed high fertility rates (NRR: 62.1-81.8%, CNRR: 67.2-98.5%).
    • Corrected NRR (CNRR) correlated significantly with semen parameters post-sperm selection (SSp), but not post-thaw (PT).
    • Higher CNRR was associated with decreased total motility, progressive spermatozoa, and abaxial implantation, and increased immotile spermatozoa (up to 47%) after SSp.

    Conclusions:

    • High frequencies of motile and abaxially implanted spermatozoa in gradient-selected sperm are unfavorable for in vivo fertility.
    • Semen quality assessment after sperm selection is a better predictor of fertility than post-thaw assessment.
    • Optimal fertility may be achieved with a specific threshold of immotile spermatozoa post-selection.