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Published on: September 16, 2013
Data on optimization of expression and purification of AIMP2-DX2 protein in Escherichia coli
Roshan Jha1, Hye Young Cho1, Ameeq Ul Mushtaq1
1College of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 30019, Republic of Korea.
Abstract:
AIMP2-DX2 is a splicing variant of AIMP2 protein which has been implicated in human lung cancer and chemoresistance of ovarian cancer (J.W. Choi, D.G. Kim, A.E. Lee, H.R. Kim, J.Y. Lee, N.H. Kwon, et al., 2011; J.W. Choi, J.W. Lee, J.K. Kim, H.K. Jeon, J.J. Choi, D.G. Kim, et al., 2012) [1,2]. We have shown, here, the data for the expression of AIMP2-DX2 protein in Escherichia coli and optimization of the critical steps in purification of AIMP2-DX2. The data described here has been successfully used to get a maximum yield of highly pure AIMP2-DX2 for subsequent characterization of its biophysical property in: "Purification and biophysical characterization of the AIMP2-DX2 protein" (R. Jha, H.Y. Cho, A. Ul Mushtaq, K. Lee, D.G. Kim, S. Kim, et al., 2017) [3].
Insights
This study details the expression and purification of the AIMP2-DX2 protein in E. coli. Optimized methods yielded highly pure AIMP2-DX2 for further biophysical characterization.
Area of Science:
- Biochemistry
- Molecular Biology
- Protein Expression
Background:
- AIMP2-DX2 is a splicing variant of the AIMP2 protein.
- AIMP2-DX2 has been linked to human lung cancer and chemoresistance in ovarian cancer.
- Previous studies indicated the involvement of AIMP2-DX2 in these diseases.
Purpose of the Study:
- To describe the expression of AIMP2-DX2 protein in Escherichia coli.
- To optimize critical purification steps for AIMP2-DX2.
- To provide data for subsequent biophysical characterization of AIMP2-DX2.
Main Methods:
- Expression of AIMP2-DX2 in E. coli.
- Optimization of protein purification protocols.
- Yield maximization and purity assessment of AIMP2-DX2.
Main Results:
- Successful expression of AIMP2-DX2 in E. coli.
- Identified and optimized critical steps for AIMP2-DX2 purification.
- Achieved maximum yield of highly pure AIMP2-DX2 protein.
Conclusions:
- The optimized purification protocol enables high-yield production of pure AIMP2-DX2.
- The generated data supports further biophysical characterization of AIMP2-DX2.
- This work facilitates future research into the role of AIMP2-DX2 in cancer.
