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Urine TREM-1 as a marker of urinary tract infection in children

Erick Sierra-Diaz1, Alejandro Bravo Cuéllar2, Pablo Cesar Ortiz Lazareno3

  • 11 Departamento de Urología, Centro Medico Nacional de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, México.

The Journal of International Medical Research
|April 4, 2017
PubMed

Insights

Triggering receptor expressed on myeloid cells (TREM)-1 is present in all children, but higher levels in urine samples from children with urinary tract infection (UTI) may indicate infection. This finding aids in UTI diagnosis.

Area of Science:

  • Pediatrics
  • Immunology
  • Infectious Diseases

Background:

  • Triggering receptor expressed on myeloid cells (TREM)-1 is a receptor implicated in infection recognition.
  • Its presence and role in pediatric urinary tract infections (UTIs) require further investigation.

Purpose of the Study:

  • To determine the presence of TREM-1 in urine samples from children with and without UTIs.
  • To assess soluble TREM-1 (sTREM-1) levels in pediatric UTI patients.

Main Methods:

  • Urine samples from 128 children were analyzed.
  • TREM-1 protein expression was detected using flow cytometry.
  • sTREM-1 levels were quantified via ELISA.

Main Results:

  • TREM-1 expression was detected in 100% of urine samples from both UTI and non-UTI groups.
  • Mean fluorescence intensity of TREM-1 differed significantly between groups (p < 0.001).
  • Elevated sTREM-1 levels were observed in children with UTIs, but not in healthy children.

Conclusions:

  • TREM-1 is universally expressed in pediatric urine samples.
  • Higher TREM-1 levels in UTI patients correlate with increased neutrophils and bacterial-induced cytokine activity.
  • TREM-1 may serve as a potential biomarker for pediatric UTIs.

Keywords:
ELISATriggering receptor expressed on myeloid cells (TREM)-1childrenflow cytometrymean fluorescence intensityneutrophilsurinary tract infection

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