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Identification and expression analysis of terminal deoxynucleotidyl transferase in humphead snapper Lutjanus
Y Huang1,2,3, J Cai1,2,3, J F Tang1,2,3
1College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, People's Republic of China.
Abstract:
A tdt gene was identified successfully from humphead snapper Lutjanus sanguineus, which contained 1710 bp encoding a protein of 463 amino acids. Results of quantitative real-time polymerase chain reaction (qRT-PCR) indicated that tdt mainly expressed in thymus and head kidney and the transcripts of tdt in these tissues were up-regulated significantly at 36 and 48 h after Vibrio harveyi infection. Meanwhile Tdt-producing cells were found in thymus and head kidney.
