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Characterization of isomeric glycan structures by LC-MS/MS
Lucas Veillon1, Yifan Huang1, Wenjing Peng1
1Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Electrophoresis
|April 4, 2017
Summary
Glycan analysis using liquid chromatography (LC) is essential for understanding glycoprotein functions. This review covers advanced LC techniques for separating complex glycans and resolving isomeric structures for mass spectrometry (MS) analysis.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Glycomics
Background:
- Glycosylation is crucial for glycoprotein regulation and function.
- Oligosaccharide complexity and ion suppression effects hinder glycan characterization.
- Chromatographic separation is vital for detailed glycan analysis, especially for isomers.
Purpose of the Study:
- To review fundamental principles of liquid chromatography (LC) techniques in glycomics.
- To highlight recent advances in LC-MS for characterizing protein glycosylation.
- To focus on methods capable of resolving isomeric glycan structures.
Main Methods:
- Review of porous graphitized carbon LC.
- Discussion of reverse phase LC in glycomics.
- Overview of ion exchange and hydrophilic interaction LC (HILIC) for glycan analysis.
Main Results:
- LC-MS is indispensable for comprehensive glycan profiling.
- Advanced LC methods offer improved separation of complex and isomeric glycans.
- Porous graphitized carbon and HILIC show promise for resolving glycan isomers.
Conclusions:
- Effective LC separation is key to advancing glycomics research.
- LC-MS techniques are critical for detailed characterization of protein glycosylation.
- Further development in LC methods will enhance the resolution of isomeric glycans.