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Detection of DT-diaphorase Enzyme with a ParaCEST MRI Contrast Agent
Iman Daryaei1, Kyle M Jones2, Mark D Pagel3
1Department of Chemistry and Biochemistry, University of Arizona, 1306 E. University Blvd., Room 221, Tucson, Arizona, 85721-0041, USA.
Chemistry (Weinheim an Der Bergstrasse, Germany)
|April 4, 2017
Abstract:
A responsive magnetic resonance (MRI) contrast agent has been developed that can detect the enzyme activity of DT-diaphorase. The agent produced different chemical exchange saturation transfer (CEST) MRI signals before and after incubation with the enzyme, NADH, and GSH at different pH values whereas it showed good stability in a reducing environment without enzyme.