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Related Concept Videos

Integrins01:10

Integrins

Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Activation of Integrins01:15

Activation of Integrins

Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Anchoring Junctions01:03

Anchoring Junctions

Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
Adherens Junctions01:24

Adherens Junctions

Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
The endothelial cells...
Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation, but...

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Related Experiment Video

Updated: Jul 1, 2026

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis
07:52

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis

Published on: December 21, 2014

α5β1 integrin mediates pulmonary epithelial cyst formation.

Susan K Legan1, Daniel D Lee2, Margaret A Schwarz2

  • 1Department of Pediatrics, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|April 4, 2017
PubMed
Summary

Alpha5 (α5) integrin is crucial for lung epithelial cyst formation by organizing extracellular matrix fibrils. Loss of α5 integrin disrupts cell polarity and cytoskeleton organization during lung development.

Keywords:
alveolarepithelial cystlung developmentpulmonary bodies

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Last Updated: Jul 1, 2026

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Extracellular Matrix Biology

Background:

  • Epithelial cyst formation requires apical-basolateral polarity established through cell interactions and extracellular matrix (ECM).
  • Alpha5 (α5) integrin plays a role in regulating epithelial cyst formation via fibronectin matrix organization and fibrillogenesis.

Purpose of the Study:

  • To investigate the role of α5 integrin in mediating epithelial cyst formation during lung development.
  • To understand how α5 integrin-mediated fibrillogenesis influences cell-cell interactions and polarity establishment.

Main Methods:

  • Utilized a three-dimensional multi-cellular self-assembly model of murine embryonic lung development.
  • Employed knockdown of α5 integrin to assess its effects on cell self-assembly, cyst formation, and polarity.

Main Results:

  • Alpha5 (α5)β1 integrin-mediated fibrillogenesis is essential for self-assembly, compaction, and epithelial cyst formation.
  • Knockdown of α5 integrin led to increased αV integrin but failed to rescue cyst formation, polarity, or cytoskeleton organization.
  • Loss of α5 integrin disrupted epithelial cell shape, apical F-actin deposition, and central epithelial localization of F-actin and Podocalyxin.

Conclusions:

  • Lung epithelial cyst formation is partly mediated by α5β1 integrin-dependent fibrillogenesis.
  • α5 integrin-mediated fibrillogenesis is critical for establishing epithelial polarity and cytoskeleton organization during lung development.