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Proteins specified by bovine herpesvirus-2.

S W Adams1, N Balachandran, L M Hutt-Fletcher

  • 1Department of Special Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610.

American Journal of Veterinary Research
|April 1, 1988
PubMed
Summary

Researchers identified 38 bovine herpesvirus type 2-induced proteins in infected bovine testes cells. These viral proteins were classified into alpha, beta, and gamma groups based on synthesis patterns.

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Area of Science:

  • Virology
  • Molecular Biology
  • Cellular Biology

Background:

  • Bovine herpesvirus type 2 (BoHV-2) is a significant pathogen affecting cattle.
  • Understanding viral protein synthesis is crucial for developing antiviral strategies.

Purpose of the Study:

  • To characterize polypeptides synthesized by bovine testes cells infected with BoHV-2.
  • To classify BoHV-2-induced proteins based on their synthesis kinetics and drug sensitivity.

Main Methods:

  • Bovine testes cells infected with BoHV-2 were labeled with [35S]methionine.
  • Proteins were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
  • Immunoprecipitation with rabbit antiserum to BoHV-2 and [3H]glucosamine labeling were performed.

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Main Results:

  • Thirty-eight virus-induced proteins (32,000–149,000 molecular weight) were detected.
  • A similar number of proteins were immunoprecipitated by anti-BoHV-2 antiserum.
  • Twelve proteins incorporated [3H]glucosamine, indicating glycosylation.
  • Proteins were classified: 3 alpha, 4 beta, 1 beta/gamma, and 27 gamma proteins.
  • Host cell protein synthesis was significantly inhibited only after 22–28 hours post-infection.

Conclusions:

  • BoHV-2 infection induces a complex array of viral proteins with distinct temporal synthesis patterns.
  • Classification into alpha, beta, and gamma groups provides insights into BoHV-2 gene expression regulation.
  • The late inhibition of host cell protein synthesis suggests a biphasic infection cycle or host response.