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Low-temperature stability of viruses in sludges
G Berg1, G Sullivan, A D Venosa
1Department of Civil and Environmental Engineering, University of Cincinnati, Ohio 45221.
Applied and Environmental Microbiology
|March 1, 1988
Summary
Enteroviruses can survive for extended periods in refrigerated sludge. This finding is crucial for understanding virus persistence in wastewater treatment and laboratory storage.
Area of Science:
- Environmental microbiology
- Virology
- Wastewater treatment
Background:
- Enteroviruses are common human pathogens found in wastewater.
- Sludge processing and storage conditions can impact pathogen survival.
- Understanding enterovirus persistence is vital for public health and environmental safety.
Purpose of the Study:
- To investigate the survival rates of enteroviruses in different types of sludge under refrigerated conditions.
- To assess the influence of sludge properties, such as pH and temperature, on enterovirus viability.
Main Methods:
- Enteroviruses were introduced into extended-aeration and oxidation ditch sludges.
- Sludge samples were maintained at 5 degrees C.
- Enterovirus survival was monitored over time.
- Sludge pH and chemical composition were characterized.
Main Results:
- Enteroviruses survived for up to 38 days in extended-aeration sludge at 5°C.
- Enteroviruses survived for up to 17 days in oxidation ditch sludge at 5°C.
- Significant enterovirus survival was observed even at a low pH of 3.5, contrary to expectations.
Conclusions:
- Refrigerated sludge can maintain high numbers of enteroviruses for extended periods.
- Low temperatures significantly enhance enterovirus survival in sludge.
- Wastewater sludge can serve as a long-term reservoir for enteroviruses, necessitating careful handling and treatment.