Related Experiment Video
Updated: Jul 11, 2026

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
Published on: November 16, 2016
Studying Factors Involved in Biogenesis of Lysobacter sp. XL1 Outer Membrane Vesicles
I V Kudryakova1, N E Suzina, N G Vinokurova
1G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences (IBPM RAS), Pushchino, Moscow Region, 142290, Russia. kudryakovairina@yandex.ru.
Abstract:
The Gram-negative bacterium Lysobacter sp. XL1 produces outer membrane vesicles that are heterogeneous in size, density, and protein composition. One of the subpopulations is secretory vesicles for lytic protease L5 of Lysobacter sp. XL1 (Kudryakova et al. (2015) FEMS Microbiol. Lett., 362, fnv137). Protein L5 was assumed to influence biogenesis of these secretory vesicles that contain it. Using a Pseudomonas fluorescens Q2-87/B expression system, it was shown that the recombinant L5 protein may act as a factor of vesicle biogenesis. This points to a possible involvement of L5 protein in Lysobacter sp. XL1 vesicle biogenesis. Furthermore, it was established that the main phospholipid of Lysobacter sp. XL1 vesicles is cardiolipin, and vesicles are formed predominantly of outer membrane regions enriched with this phospholipid. This indicates that cardiolipin participates in biogenesis of all vesicle subpopulations in Lysobacter sp. XL1.
Related Concept Videos
Intralumenal Vesicles and Multivesicular Bodies
Outer Layers of the Cell Envelope
Biosynthesis of Lipids
Formation of Lipopolysaccharides

