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Updated: Jul 29, 2026

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy
Published on: October 13, 2014
REVERSIBLE COMPRESSION OF DEHYDRATED PEAS
A R Rahman1, S Bishov1, D E Westcott1
1U. S. Army Natick Laboratories, Natick, Mass. 01760 U. S. A.
Abstract:
Freeze-dried peas were compressed 4-fold and subsequently rehydrated to virtually their precompression characteristics. Histological studies showed that conventional processes of freezing and freeze-drying result in considerable disruption of cell walls. Contrary to what was anticipated, compression forces of 1000-2000 psi resulted in only a slight increase in breakage of cell walls. However, no correlation was established between cell disruption or texture and increasing compression force. A study of the effects of plasticizing moisture and compression force enabled optimization of processing conditions resulting in compressed peas which can be reconstituted to virtually their precompression characteristics.

