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Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
Published on: December 4, 2017
Iron-sulfur centers involved in methanogenic electron transfer in Methanobacterium thermoautotrophicum (delta-H)
K R Rogers1, K Gillies, J R Lancaster
1Department of Chemistry and Biochemistry, Utah State University, Logan 84322-0300.
Abstract:
Utilizing a subcellular particulate preparation from Methanobacterium thermoautotrophicum (delta-H) which contains all detectable methanogenic electron transfer activity, we present the results of the effects of the anaerobic addition of oxidized factor F420 and of methyl coenzyme M plus ATP on the EPR signals from reduced iron-sulfur centers and a rapidly-relaxing radical species. Based on these results, we report the existence of a minimum of three iron-sulfur centers which are capable of donating electrons to these cofactors.
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