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Updated: Aug 21, 2026
ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
Published on: October 23, 2009
Determination of mycotoxins in plant-based beverages using QuEChERS and liquid chromatography-tandem mass
Eugènia Miró-Abella1, Pol Herrero2, Núria Canela2
1Department of Analytical Chemistry and Organic Chemistry, Universitat Rovira i Virgili, Sescelades Campus, Marcel·lí Domingo, s/n, 43007 Tarragona, Spain; Group of Research on Omic Methodologies (GROM), Centre for Omic Sciences (COS), Universitat Rovira i Virgili, Av. Universitat 1, 43204 Reus, Spain.
Abstract:
A method was developed for the simultaneous determination of 11 mycotoxins in plant-based beverage matrices, using a QuEChERS extraction followed by ultra-high performance liquid chromatography coupled to tandem mass spectrometry detection (UHPLC-(ESI)MS/MS). This multi-mycotoxin method was applied to analyse plant-based beverages such as soy, oat and rice. QuEChERS extraction was applied obtaining suitable extraction recoveries between 80 and 91%, and good repeatability and reproducibility values. Method Quantification Limits were between 0.05μgL-1 (for aflatoxin G1 and aflatoxin B1) and 15μgL-1 (for deoxynivalenol and fumonisin B2). This is the first time that plant-based beverages have been analysed, and certain mycotoxins, such as deoxynivalenol, aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, ochratoxin A, T-2 toxin and zearalenone, were found in the analysed samples, and some of them quantified between 0.1μgL-1 and 19μgL-1.
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