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Related Experiment Videos

Microbiota and Human Health: characterization techniques and transference.

Rosa Del Campo-Moreno1, Teresa Alarcón-Cavero2, Giuseppe D'Auria3

  • 1Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, España; Red Española de Investigación en Patología Infecciosa (REIPI), Sevilla, España.

Enfermedades Infecciosas Y Microbiologia Clinica (English Ed.)
|April 5, 2017
PubMed
Summary

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Correction: Villalva et al. Antioxidant, Anti-Inflammatory, and Antibacterial Properties of an <i>Achillea millefolium</i> L. Extract and Its Fractions Obtained by Supercritical Anti-Solvent Fractionation against <i>Helicobacter pylori</i>. <i>Antioxidants</i> 2022, <i>11</i>, 1849.

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The human microbiota, encompassing beneficial and harmful microbes, is better understood thanks to 16S rRNA sequencing. This technology reveals microbial roles in health and disease, including treatments like fecal microbiota transplant.

Area of Science:

  • Microbiology
  • Human Health
  • Genomics

Background:

  • The human body hosts a vast community of microorganisms known as the microbiota.
  • Microbiota members are classified as commensals, mutualists, or pathogens based on their interactions.
  • Understanding the microbiota's role is crucial for human health research.

Purpose of the Study:

  • To detail human bacterial ecosystems and their links to various diseases.
  • To describe the fecal microbiota transplant (FMT) procedure for conditions like recurrent Clostridium difficile infection.
  • To explain the methodological basis of advanced molecular techniques for microbiota characterization.

Main Methods:

  • 16S rRNA next-generation sequencing (16S rDNA gene) has revolutionized microbiota analysis.
Keywords:
Faecal microbiota transplantHuman microbiotaInfluence on healthInfluencia en la saludMicrobiota humanaNext generation sequencingNext generation wequencingTrasferencia de materia fecal

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  • Molecular techniques provide detailed insights into microbial community composition.
  • Fecal microbiota transplantation is a key therapeutic application discussed.

    • Main Results:

    • Significant advancements in understanding microbiota composition and function.
    • Established scientific evidence linking specific microbiota profiles to different diseases.
    • FMT demonstrates efficacy in treating specific gastrointestinal disorders.

    Conclusions:

    • 16S rRNA sequencing is a powerful tool for studying the human microbiota.
    • The microbiota plays a critical role in maintaining human health and disease pathogenesis.
    • Further research into microbiota-based diagnostics and therapeutics is warranted.